Composite

Part:BBa_K5269024:Design

Designed by: Yuichiro Nakai   Group: iGEM24_TokyoTech   (2024-09-28)


SpyTag-Glucanase-His-Tag and BsmB I cut site(for GGA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 235
    Illegal NotI site found at 1603
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2845
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 627
    Illegal NgoMIV site found at 1252
    Illegal NgoMIV site found at 1995
    Illegal NgoMIV site found at 2620
    Illegal AgeI site found at 385
    Illegal AgeI site found at 1753
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

References